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Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.

Optics letters (1994-06-01)
S W Hell, J Wichmann
RESUMEN

We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function. In contrast to near-field scanning optical microscopy, this method can produce three-dimensional images of translucent specimens.

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Abberior® STAR 580, NHS ester, for STED application
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Abberior® STAR 635, NHS ester, for STED application
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Abberior® FLIP 565, maleimide, for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)
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Abberior® STAR 512, maleimide, for STED application