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50536-U

Ascentis^® Express C18, 5 μm HPLC Column

Name: Ascentis<SUP>®</SUP> Express C18, 5 μm HPLC Column
Brand: SUPELCO

5 μm particle size, L × I.D. 10 cm × 4.6 mm

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About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

Ascentis^®

feature

endcapped

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

10 cm × 4.6 mm

surface area

90 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

90 Å pore size

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis^® Express 5 μm C18 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The Fused-Core^® particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the highly uniform overall particle size of 5-microns. The densely bonded, extensively endcapped dimethyloctadecyl stationary phase of Ascentis Express 5 μm C18 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Application

HPLC Analysis of Amine Medications on Ascentis^® Express C18, 5 μm

HPLC Analysis of Pyrethrins on Ascentis^® Express C18, 5 μm

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Determination of tropane alkaloids atropine and scopolamine by liquid chromatography-mass spectrometry in plant organs of Datura species.

Silvia Jakabová et al.

Journal of chromatography. A, 1232, 295-301 (2012-03-07)

Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a

High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation.

Judy Stone

Methods in molecular biology (Clifton, N.J.), 1378, 301-320 (2015-11-26)

Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 %

Bioequivalance and pharmacokinetic study of febuxostat in human plasma by using LC-MS/MS with liquid liquid extraction method.

Babu Rao Chandu et al.

SpringerPlus, 2(1), 194-194 (2013-06-07)

A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human

Fast gradient HPLC/MS separation of phenolics in green tea to monitor their degradation.

Petra Šilarová et al.

Food chemistry, 237, 471-480 (2017-08-03)

The degradation of catechins and other phenolics in green tea infusions were monitored using fast HPLC/MS separation. The final separation was performed within 2.5min using Ascentis Express C18 column (50mm×2.1mm i.d.) packed with 2μm porous shell particles. Degradation was studied

Development and Validation of an LC-MS-MS Method for Determination of Simvastatin and Simvastatin Acid in Human Plasma: Application to a Pharmacokinetic Study.

Pankaj Partani et al.

Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)

A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

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Artículos

HPLC Analysis of Pain Relievers on Ascentis® Express C18

Separation of Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder; Caffeine, meets USP testing specifications, anhydrous; Benzoic acid, meets USP testing specifications; Aspirin, meets USP testing specifications

Protocolos

USP HPLC Analysis of Ampicillin Sodium on Ascentis® Express C18

USP HPLC Analysis of Ampicillin Sodium on Ascentis^® Express C18

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