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Merck
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SML0430

Sigma-Aldrich

PR-619

≥95% (HPLC)

Sinónimos:

2,6-Diamino-3,5-dithiocyanopyridine, Thiocyanic acid C,C′-(2,6-diamino-3,5-pyridinediyl) ester; 2,6-Diaminopyridine-3,5-bis(thiocyanate)

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About This Item

Fórmula empírica (notación de Hill):
C7H5N5S2
Número de CAS:
2645-32-1
Peso molecular:
223.28
MDL number:
MFCD00830384
UNSPSC Code:
12352200
PubChem Substance ID:
329825425
NACRES:
NA.77

assay

≥95% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 5 mg/mL (clear solution)

storage temp.

−20°C

SMILES string

Nc1nc(N)c(SC#N)cc1SC#N

InChI

1S/C7H5N5S2/c8-2-13-4-1-5(14-3-9)7(11)12-6(4)10/h1H,(H4,10,11,12)

InChI key

ZXOBLNBVNROVLC-UHFFFAOYSA-N

Application

PR-619 has been used:
  • as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells
  • as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction
  • in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays

Biochem/physiol Actions

PR-619 is a cell permeable broad spectrum deubiquitylating enzymes (DBUs) inhibitor. PR-619 induces the accumulation of polyubiquitylated proteins in cells without directly affecting proteasome activity.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Large-scale identification of ubiquitination sites by mass spectrometry
Udeshi ND, et al.
Nature Protocols, 8(10), 1950-1950 (2013)
Tyler Bland et al.
Endocrinology, 160(8), 1982-1998 (2019-06-15)
Leptin has neurotrophic actions in the hippocampus to increase synapse formation and stimulate neuronal plasticity. Leptin also enhances cognition and has antidepressive and anxiolytic-like effects, two hippocampal-dependent behaviors. In contrast, mice lacking leptin or the long form of the leptin
Cytosolic iron-sulfur assembly is evolutionarily tuned by a cancer-amplified ubiquitin ligase
Weon JL, et sl.
Molecular Cell, 69(1), 113-125 (2018)
Anne Garreau et al.
Journal of immunology (Baltimore, Md. : 1950), 199(8), 2758-2766 (2017-09-08)
Themis is a new component of the TCR signaling machinery that plays a critical role during T cell development. The positive selection of immature CD4
Ian G Cowell et al.
Molecular pharmacology, 96(5), 562-572 (2019-09-14)
2,6-Diaminopyridine-3,5-bis(thiocyanate) (PR-619) is a broad-spectrum deubiquitinating enzyme (DUB) inhibitor that has been employed in cell-based studies as a tool to investigate the role of ubiquitination in various cellular processes. Here, we demonstrate that in addition to its action as a
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