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Merck

F5554

Sigma-Aldrich

Formalin, 10%, Neutral Buffered, Wintergreen Scented

histological fixative, pH 6.9-7.1

Sinónimos:

Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

shelf life

Expiry date on the label.

color

colorless

pH

6.90-7.10

application(s)

hematology
histology

storage temp.

room temp

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Application

Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
Donnenberg VS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 83(1), 141-149 (2013)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
Neither epithelial nor mesenchymal circulating tumor cells isolated from breast cancer patients are tumorigenic in NOD-scid Il2rg null mice
Donnenberg VS, et al.
NPJ breast cancer, 2, 16004-16004 (2016)
p38alpha MAP kinase as a sensor of reactive oxygen species in tumorigenesis
Dolado I, et al.
Cancer Cell, 11(2), 191-205 (2007)
Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)

Protocolos

Cultrex® 3-D Culture Matrix™ RGF BME is the first Basement Membrane Extract produced and qualified specifically for use in 3D culture studies.

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