D225
4,5-Diaminofluorescein diacetate solution
5 mM in DMSO, ≥97% (HPLC)
Sinónimos:
DAF-2 DA
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About This Item
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Quality Level
assay
≥97% (HPLC)
form
solution
availability
not available in Japan
concentration
5 mM in DMSO
shipped in
wet ice
storage temp.
−20°C
General description
4,5-Diaminofluorescein diacetate (DAF-2 DA) solution is a membrane-permeable fluorescent probe, which measures nitric oxide (NO) in living cells. It is used to study the synthesis of NO in bovine aortic endothelial cells (BAECs). DAF-2 DA imaging shows that ciliated epithelial cells produces high amount of NO than non-epithelial inflammatory cells.
Application
Cell-permeable fluorescent detector of nitric oxide in living cells.
Legal Information
DAF-2 is manufactured by Daiichi Pure Chemicals Co., LTD.
US Patent No. 5,874,590
US Patent No. 5,874,590
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Determinants of shear stress-stimulated endothelial nitric oxide production assessed in real-time by 4, 5-diaminofluorescein fluorescence
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Nitric oxide (NO) is a regulatory molecule in the vascular system and its inhibition due to endothelial injury contributes to cardiovascular disease. The glycocalyx is a thin layer of glycolipids, glycoproteins, and proteoglycans on the surface of mammalian epithelial cells.
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Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the
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