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Merck

W2261

Millipore

WL nutrient broth

suitable for microbiology, NutriSelect® Plus

Sinónimos:

Wallerstein Laboratory Nutrient Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
food and beverages

microbiology

suitability

selective for Candida spp.
selective for Escherichia coli
selective for Lactobacillus spp.
selective for Pichia spp.
selective for Proteus spp.
selective for Saccharomyces spp.
selective for Zygosaccharomyces spp.
selective for coliforms

Application

Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022

Preparation Note

Suspend 60.25 g of WL Nutrient Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. If desired to obtain a pH of 6.5 add a 1% solution of sodium bicarbonate (Cat. No. 71627).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Artículos

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

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