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11207750910

Roche

Anti-Digoxigenin-Rhodamine, Fab fragments

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

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About This Item

UNSPSC Code:
12352203

biological source

sheep

Quality Level

100

conjugate

rhodamine conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized (clear, red solution after reconstitution)

packaging

pkg of 200 μg

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

Categorías relacionadas

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Specificity

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
  • DNA fiber assay

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
  • Immunohistocytochemistry: 20 to 50 μg/ml
  • In situ hybridization: 20 to 50 μg/ml

Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.

Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Spencer W Luebben et al.
Nucleic acids research, 41(22), 10283-10297 (2013-09-06)
HELQ is a superfamily 2 DNA helicase found in archaea and metazoans. It has been implicated in processing stalled replication forks and in repairing DNA double-strand breaks and inter-strand crosslinks. Though previous studies have suggested the possibility that HELQ is
Galina Pendinen et al.
Genome, 51(9), 714-720 (2008-09-06)
Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
Olga G Silkova et al.
Plants (Basel, Switzerland), 10(10) (2021-10-24)
To date, few data have been accumulated on the contribution of meiotic restitution to the formation of Triticum aestivum hybrid karyotypes. In this study, based on FISH and C-banding, karyotype reorganization was observed in three groups of F5 wheat-rye hybrids
Shin-Ichiro Takebayashi et al.
Epigenetics & chromatin, 6(1), 42-42 (2013-12-18)
Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties. Here we show that
Xiaochun Wu et al.
The FEBS journal, 285(21), 3945-3958 (2018-09-07)
The krüppel-like factors (KLFs) are a family of transcription factor proteins that regulate a wide range of biological processes. In an RNAi-based screening experiment, we identified dendritic arbor reduction 1 (Dar1), which is a KLF member in Drosophila, that inhibited
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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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