MABT335

Anti-Desmoglein-3, clone 5G11 Antibody

clone 5G11, from mouse

• Sinónimos: Desmoglein-3, 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5G11, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin)
• isotype: IgG1κ
• NCBI accession no.: NP_001935
• UniProt accession no.: P32926
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DSG3(1830)

Descripción

General description

Desmoglein-3 (DSG3) is a calcium binding membrane protein that is localized desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points. Desmosomes are cell-cell junctions between epithelial, myocardial and other cells types. Strong intercellular adhesion is critical for tissues that experience mechanical stress, such as the skin and heart. Desmosomes provide adhesive strength to tissues by anchoring desmosomal cadherins of neighboring cells to the intermediate filament cytoskeleton. It is thought that raft membrane microdomains play a role in desmosome homeostasis by regulating the dynamics of desmosome assembly and disassembly. In human keratinocytes, Desmoglein-3 (DSG3) is raft associated and disruption of rafts prevents desmosome assembly. Desmoglein-3 is one of four sister proteins in the desmoglein family. Desmoglein-3 is also the autoantigen for Pemphigus vulgaris (PV) a lethal skin disease that is a result of autoantibodies against Desmoglein-3.

Immunogen

Recombinant protein corresponding to human Desmoglein-3.

Application

Immunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in human scalp hair follicles and epidermal inclusions (Wu, H., et al. (2003). J Invest Dermatology. 120(6):1152-1157).
Immunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in in human adult skin tissue (Wu, H., et al. (2013). New England Journal of Med. 343(1):31-35).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Desmoglein-3, clone 5G11 Antibody is validated for use in Immunohistochemistry (Paraffin) for the detection of Desmoglein-3.

Quality

Evaluated by Immunohistochemistry in human tonsil and human skin tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Desmoglein-3 in human tonsil and human skin tissue.

Target description

107 kDa calculated

Linkage

Replaces: MABT470

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids