648493-M

TRPML Agonist, ML-SA1

The TRPML Agonist, ML-SA1 controls the biological activity of TRPML. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Sinónimos: TRPML Agonist, ML-SA1, 2-(2-Oxo-2-(2,2,4-trimethyl-3,4-dihydroquinolin-1(2H)-yl)ethyl)isoindoline-1,3-dione, Mucolipin Synthetic Agonist 1, ML-SA1
• Fórmula empírica (notación de Hill): C₂₂H₂₂N₂O₃
• Número de CAS: 332382-54-4
• Peso molecular: 362.42
• UNSPSC Code: 12352200

Propiedades

• Quality Level: 100
• assay: ≥97% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: DMSO: 25 mg/mL
• storage temp.: 2-8°C
• InChI: 1S/C22H22N2O3/c1-14-12-22(2,3)24(18-11-7-6-8-15(14)18)19(25)13-23-20(26)16-9-4-5-10-17(16)21(23)27/h4-11,14H,12-13H2,1-3H3
• InChI key: KDDHBJICVBONAX-UHFFFAOYSA-N

Descripción

General description

A membrane permeable dihydroquinolinyloxo-isoindolinedione compound that acts as a potent and selective agonist of lysosomal mucolipin transient receptor potential (TRP) channel 1,2,3 (TRPML) in all mammalian cells. Does not appear to affect six other related TRP channels. Shown to rapidly activate whole-endolysosome TRPML-like current and induce lysosomal Ca²⁺ release (~10 µM). Its activating action on whole-endolysosome is comparable to that of PI(3,5)P2 (1 µM) with which it shows a synergistic effect. ML-SA1-induced Ca²⁺ responses are reportedly abolished by glycyl-L-phenylalanine 2-naphthylamide, induces osmotic lysis of lysosomes, and Bafilomycin A1 (>Cat. No. 196000), a V-ATPase inhibitor. Also reduces lactosylceramide and cholesterol accumulation and corrects trafficking defects in Niemann-Pick (NP) disease cells.

Biochem/physiol Actions

Primary Target
TRPML1,2,3

Warning

Toxicity: Standard Handling (A)

Other Notes

Shen, D., et al. 2012. Nat. Commun.3, 731.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable