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Key Documents

14-557-M

Sigma-Aldrich

B-Raf (V599E) Protein, active, 10 µg

Active, N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 67.3 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

General description

N-terminal GST-tagged recombinant human B-Raf residues 416-end, containing a V599E mutation (also designated V600E)
Product Source: Expressed by baculovirus in Sf21 insect cells

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: B-Raf
Target Sub-Family: TKL

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-557M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of MBP substrate in vitro

Physical form

Glutathione agarose affinity chromatography

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mathew J Garnett et al.
Cancer cell, 6(4), 313-319 (2004-10-19)
RAF proteins are components of a conserved signaling pathway that regulates cellular responses to extracellular signals. Despite over 20 years of research into the regulation and function of the RAF proteins, it was only realized recently that the B-RAF isoform
Stevan R Hubbard
Cell, 116(6), 764-766 (2004-03-24)
A study by Wan et al. in this issue of Cell demonstrates that the majority of oncogenic mutations in the B-Raf protein kinase result in increased catalytic activity, through disruption of the autoinhibited state of the kinase domain. Surprisingly, several
Kathryn E Mercer et al.
Biochimica et biophysica acta, 1653(1), 25-40 (2003-06-05)
A recent report has shown that activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations represent a single nucleotide change

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