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Merck
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Key Documents

07-1218

Sigma-Aldrich

Anti-PP1γ1 Antibody

Upstate®, from rabbit

Sinónimos:

Protein phosphatase 1C catalytic subunit, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, Protein phosphatase 1, catalytic subunit, gamma isoform, Serine/threonine phosphatase 1 gamma

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

bovine, mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002710.1

UniProt accession no.

P36873

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PPP1CC(5501)

General description

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.

Specificity

Recognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.

Immunogen

Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).

Application

Anti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.
Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Quality

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.

Target description

~37 kDa

Linkage

Replaces: AB4084

Physical form

Format: Purified
Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa Cell Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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American journal of physiology. Renal physiology, 305(3), F362-F369 (2013-05-31)
Activation of the transcription factor NFAT5 by high NaCl involves changes in phosphorylation. By siRNA screening, we previously found that protein targeting to glycogen (PTG), a regulatory subunit of protein phosphatase1 (PP1), contributes to regulation of high NaCl-induced NFAT5 transcriptional
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