Skip to Content
Merck
All Photos(2)

Key Documents

SRP0415

Sigma-Aldrich

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):

OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

CAS Number:
1644286-11-2
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

59 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

1.8 mg/mL

NCBI accession no.

NM_182470

UniProt accession no.

P14618

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PKM(5315)

General description

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2. PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues, retina, adipose tissues, and islets. The PKM1 gene is located on the human chromosome at 15q23.

Application

PKM1active human has been used in a cell-based kinase assay to study the ability ofa compound to inhibit the kinase activity of PKM1. It has also been used inoxidative stress experiments on PKM2.

Biochem/physiol Actions

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

C Parkison et al.
Biochemical and biophysical research communications, 179(1), 668-674 (1991-08-30)
Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and
Rumeysa Biyik-Sit et al.
Cancers, 13(16) (2021-08-28)
An elevated expression of phosphoserine aminotransferase 1 (PSAT1) has been observed in multiple tumor types and is associated with poorer clinical outcomes. Although PSAT1 is postulated to promote tumor growth through its enzymatic function within the serine synthesis pathway (SSP)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service