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Key Documents

IgH-2

90030804, lizard cardiovascular , Epithelial-like

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About This Item

UNSPSC Code:
41106514

product name

IgH-2, 90030804

biological source

lizard cardiovascular

growth mode

Adherent

karyotype

2n = 34

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Iguana heart

Cell Line Description

From the heart of a normal immature male Iguana. Cells can support the replication of herpes simplex, PRV, vaccinia and Iguana viruses at 36°C. The only reported reptilian line to be established and serially propagated at an incubation temperature as high as 36°C.

Application

Virus replication studies: HSV, PRV, vaccinia and Iguana viruses

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 36°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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