MABT126

Anti-Obscurin Antibody, clone 5H10A

clone 5H10A, from mouse

• Synonym(s): EC:2.7.11.1, Obscurin-RhoGEF, Obscurin-myosin light chain kinase, Obscurin-MLCK
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5H10A, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001092093
• UniProt accession no.: Q5VST9
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... OBSCN(84033)

Description

General description

Obscurin (UniProt: Q5VST9; also known as EC:2.7.11.1, Obscurin-RhoGEF, Obscurin-myosin light chain kinase, Obscurin-MLCK) is encoded by the OBSCN (also known as KIAA1556, KIAA1639) gene (Gene ID: 84033) in human. Obscurin is a member of the family of giant sarcomeric signaling proteins that includes titin and nebulin. It is expressed in cardiac and skeletal muscle, and plays a role in the organization of myofibrils during sarcomere assembly. Obscurin contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. The dominant location of obscurin in mature myofibrils is shown to be at the peripheries of Z-disks and M-lines, where its position allows it to communicate with the surrounding myoplasm. Obscurin is reported to associates with the contractile apparatus through its binding to titin, sarcomeric myosin, and perhaps other proteins of the contractile apparatus. Obscurin is tightly associated with the mature contractile apparatus, hence it displays only limited solubility under non-denaturing conditions. However, it can be extracted from adult muscle fibers only in the presence of 2% SDS and 1% beta-mercaptoethanol. It is shown that primary cultures of neonatal skeletal myotubes contain large amounts of the 800 kDa obscurin isoform that can be solubilized with neutral detergents during early stages of sarcomere formation. (Ref.: Kontrogianni-Konstantopoulos, A., and Bloch, RJ. (2005). J Muscle Res Cell Motil. 26(6-8):419-26).

Specificity

Clone 5H10A detects obscurin in heart and skeletal muscle.

Immunogen

Epitope: N-terminus

GST-tagged full-length human recombinant Obscurin.

Application

Research Category
Cell Structure

This mouse monoclonal Anti-Obscurin, clone 5H10A Antibody, Cat. No. MABT126 is tested in Immunofluorescence Immunohistochemistry, and Western Blotting for the detection of obscurin.

Western Blotting Analysis: A representative lot detected Obscurin in Western Blotting applications (Bowman, A.L., et. al. (2007). FEBS Lett. 581(8):1549-54; Ackermann, M.A., et. al. (2014). PLoS One. 9(2):e88162).

Immunofluorescence Analysis: A representative lot detected Obscurin in Immunofluorescence applications (Bowman, A.L., et. al. (2007). FEBS Lett. 581(8):1549-54).

Immunofluorescence Analysis: A representative lot detected Obscurin in mouse heart left ventricle and mouse quadriceps muscle (Courtesy of Li-Yen R. Hu, Ph.D., and Aiketerini Kontrogianni-Konstantopoulos, Ph.D. , University of Maryland, School of Medicine, USA).

Immunohistochemistry Analysis: A representative lot detected Obscurin in Immunohistochemistry applications (Kontrogianni-Konstantopoulos, A., et. al. (2006). FASEB J. 20(12):2102-11; Ackermann, M.A., et. al. (2014). PLoS One. 9(2):e88162).

Western Blotting Analysis: A representative lot detected Obscurin in mouse heart left ventricle and mouse tibialis anterior muscle tissues (Courtesy of Li-Yen R. Hu, Ph.D., and Aiketerini Kontrogianni-Konstantopoulos, Ph.D. , University of Maryland, School of Medicine, USA).

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Target description

~800 kDa observed; 868.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable