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Key Documents

05-1470

Sigma-Aldrich

Anti-Raptor Antibody, clone 1H6.2

clone 1H6.2, from mouse

Synonym(s):

P150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6.2, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_065812.1

UniProt accession no.

Q8N122

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RPTOR(57521)
mouse ... Rptor(74370)

General description

Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.

Specificity

This antibody recognizes Raptor.

Immunogen

Epitope: Unknown
Recombinant protein corresponding to human Raptor.

Application

Anti-Raptor Antibody, clone 1H6.2 detects level of Raptor & has been published & validated for use in WB, IP & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Raptor in NIH/3T3 cells.

Immunoprecipitation Analysis: 20 µg from a previous lot immunoprecipitated Raptor in HEK293 cell lysate.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Raptor on 10 µg of HEK 293 cell lysate.

Target description

~ 150 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK 293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Host mTORC1 signaling regulates andes virus replication.
McNulty, S; Flint, M; Nichol, ST; Spiropoulou, CF
Journal of virology null
Julia Dyachok et al.
The Journal of biological chemistry, 291(43), 22414-22426 (2016-09-03)
The mechanistic target of rapamycin complex 1 (mTORC1) coordinates cell growth with its nutritional, hormonal, energy, and stress status. Amino acids are critical regulators of mTORC1 that permit other inputs to mTORC1 activity. However, the roles of individual amino acids
Milagros Suarez et al.
Oncotarget, 12(10), 955-966 (2021-05-21)
The MAPK-interacting kinases 1 and 2 (MNK1/2) have generated increasing interest as therapeutic targets for acute myeloid leukemia (AML). We evaluated the therapeutic potential of the highly-selective MNK1/2 inhibitor Tomivosertib on AML cells. Tomivosertib was highly effective at blocking eIF4E
Jing Lu et al.
Blood, 122(9), 1610-1620 (2013-07-03)
Multiple myeloma (MM) is the second most common hematologic malignancy. Despite recent treatment advances, it remains incurable. Here, we report that Pim2 kinase expression is highly elevated in MM cells and demonstrate that it is required for MM cell proliferation.
Shan Ma et al.
Experimental eye research, 135, 1-13 (2015-04-19)
Cones are the primary photoreceptor (PR) cells responsible for vision in humans. They are metabolically highly active requiring phosphoinositide 3-kinase (PI3K) activity for long-term survival. One of the downstream targets of PI3K is the kinase mammalian target of rapamycin (mTOR)
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