P3563
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
Synonym(s):
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
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About This Item
UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25
Recommended Products
form
powder
Quality Level
solubility
water: soluble, clear, colorless (1 pouch dissolved in 1L)
application(s)
cell analysis
diagnostic assay manufacturing
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General description
Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
Application
Phosphate buffered saline has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA)
- telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
- cell isolation
Packaging
Foil pouches
Reconstitution
Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
Other Notes
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Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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