Recommended Products
grade
for molecular biology
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
pT7-FLAG™-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a N-terminal Met-FLAG fusion protein containing the FLAG® epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.
Application
pT7-FLAG®-1 Expression Vector has been used in the cloning of ubiquitin fusion protein and connexin 43 (Cx43) C-terminal fragment.
Components
pT7-FLAG™-1 Expression Vector (P6742) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pT7-FLAG™-1-BAP Control Vector (P6992) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pT7-FLAG™-1-BAP Control Vector (P6992) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC
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Storage Class Code
12 - Non Combustible Liquids
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Chase T Archer et al.
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