HPLC Analysis of Sugars on Ascentis® Si
CONDITIONS
column
Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase
[A] water; [B] acetonitrile; (15:85, A:B)
flow rate
1.0 mL/min
column temp.
35 °C
detector
ELSD, 55 °C, 3.5 bar nitrogen
injection
10 μL
Description
Analysis Note
This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany