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A novel acetylcholine bioensor and its electrochemical behavior.

Journal of biomedical nanotechnology (2013-04-30)
Wei Wang, Yan Deng, Song Li, Hongna Liu, Zhuoxuan Lu, Liming Zhang, Lin Lin, Lijian Xu
ABSTRACT

A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping beta-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATChI). It was shown that the modified nano-PPCPE displays a high selectivity and sensitivity. The acetylcholine biosensor showed the linear range from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetylcholine chloride, suitable for cell culture
Sigma-Aldrich
Acetylcholine chloride, ≥99% (TLC)
Sigma-Aldrich
Acetylcholine chloride, pkg of 150 mg (per vial)
Sigma-Aldrich
Acetylcholine chloride, ≥99% (TLC), free-flowing, Redi-Dri
Sigma-Aldrich
Acetylcholine iodide, ≥97%
Sigma-Aldrich
Acetylcholine bromide, ≥99%