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H4652

Sigma-Aldrich

HMG-1 human

lyophilized powder, ≥90% (SDS-PAGE), Histidine-tagged, recombinant, expressed in E. coli

Synonym(s):

Amphoterin, HMGB1, High-Mobility-Group protein

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About This Item

MDL number:
MFCD03457085
UNSPSC Code:
12352202
PubChem Substance ID:
24895651

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

mol wt

25 kDa (215 amino acids; comprises two homologous HMG boxes, rich in basic amino acids, and an acidic tail at the carboxy-terminus)

UniProt accession no.

P09429

shipped in

dry ice

storage temp.

−20°C

SMILES string

COC(=O)CCCCCCCCCCCCCCO

Gene Information

human ... HMGB1(3146)

Related Categories

General description

HMG-1 (high mobility group protein) is a nuclear protein of molecular weight 30kDa. It is secreted from macrophages, monocytes and necrotic cells. It is ubiquitously expressed. The protein has basic HMG (high mobility group) box domains and an acidic carboxyl-terminus.

Application

HMG-1 (high-mobility-group protein) human has been used:
  • to prepare a standard curve for measuring HMG1 using ELISA (enzyme-linked immunosorbent assay) test
  • to study the apoptotic ratios of Lewis cells in presence of exogenous HMG-1
  • to study the changes in Th (T helper)-17 cell expansion in the presence of HMG-1 under in vitro conditions
  • for the migration assay of GM7373 cells

Biochem/physiol Actions

HMG-1 (high mobility group protein) is a non-histone chromosomal protein, which promotes the sequence specific DNA attachment of proteins. HMG1 binds DNA in a non-sequence specific manner. It is involved in DNA replication, transcription and repair. In addition, it is up-regulated in cancers and is associated with cellular growth, vasculogenesis and apoptosis regulation. In the presence of infection or injury, HMG1 is secreted and mediates inflammation. It is associated with the pathogenesis of autoimmune diseases, such as rheumatoid arthritis (RA), inflammatory bowel disease (IBD) and systemic lupus erythematosus (SLE).

Physical form

Lyophilized powder containing HEPES buffer salts, pH 7.8, NaCl, EDTA, Triton X-100, Leupeptin and AEBSF.

Analysis Note

Tested in DNA retardation assay

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H318 - H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Taguchi et al.
Nature, 405(6784), 354-360 (2000-06-01)
The receptor for advanced glycation end products (RAGE), a multi-ligand member of the immunoglobulin superfamily of cell surface molecules, interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. Engagement of
X Xu et al.
Scandinavian journal of immunology, 79(6), 386-394 (2014-03-29)
Upregulated high-mobility group box 1 (HMGB1) has been found in many diseases. Nevertheless, the function of HMGB1 on modulating the proliferation of lung cancer cells (Lewis cells) and inhibiting apoptosis is poorly understood, as well as the involved intracellular signalling.
Tian-Tian Ma et al.
PloS one, 10(4), e0123586-e0123586 (2015-04-18)
High mobility group box-1 (HMGB1), a kind of pro-inflammatory mediator, is associated with inflammatory conditions and tissue damage. Our previous study demonstrated that the circulating levels of HMGB1 correlated with disease activity of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). In
H Wang et al.
Science (New York, N.Y.), 285(5425), 248-251 (1999-07-10)
Endotoxin, a constituent of Gram-negative bacteria, stimulates macrophages to release large quantities of tumor necrosis factor (TNF) and interleukin-1 (IL-1), which can precipitate tissue injury and lethal shock (endotoxemia). Antagonists of TNF and IL-1 have shown limited efficacy in clinical
Julia Matena et al.
International journal of molecular sciences, 16(4), 7478-7492 (2015-04-08)
To improve well-known titanium implants, pores can be used for increasing bone formation and close bone-implant interface. Selective Laser Melting (SLM) enables the production of any geometry and was used for implant production with 250-µm pore size. The used pore
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