MABS421
Anti-DKK3, clone 4G7 Antibody
ascites fluid, clone 4G7, from mouse
Synonym(s):
Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(2)
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Related Categories
General description
Dickkopf-related protein 3, also known as Dickkopf-3, or Dkk-3, or hDkk-3, and encoded by the gene DKK3/ REIC is a member of the dickkopf family. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. This secreted protein is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.
Immunogen
Purified recombinant fragment of human DKK3 expressed in E. Coli
Application
Anti-DKK3, clone 4G7 Antibody is an antibody against DKK3 for use in western blotting & flow cytometry.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DKK3 in MCF-7 cells.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
Evaluated by Western Blotting in HEK293, MCF-7 and HL7702 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DKK3 in HEK293, MCF-7 and HL7702 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DKK3 in HEK293, MCF-7 and HL7702 cell lysates.
Target description
~44 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service