341220

Exo1

A cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells by inducing tubulation and collapsing of the Golgi membrane.

• Synonym(s): Exo1, 2-(4-Fluorobenzoylamino)methylbenzoate, 2-(4-Fluorobenzoylamino)benzoic Acid Methyl Ester, ER Export Inhibitor I
• Empirical Formula (Hill Notation): C₁₅H₁₂FNO₃
• CAS Number: 75541-83-2
• Molecular Weight: 273.26
• MDL number: MFCD00028104
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMF: 25 mg/mL; DMSO: 25 mg/mL; ethanol: 5 mg/mL; methanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C15H12FNO3/c1-20-15(19)12-4-2-3-5-13(12)17-14(18)10-6-8-11(16)9-7-10/h2-9H,1H3,(H,17,18)
• InChI key: KIAPWMKFHIKQOZ-UHFFFAOYSA-N

Description

General description

A cell-permeable methylanthranilate analog that reversibly blocks vesicular traffic from the ER to the Golgi in mammalian cells (IC₅₀ = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and re-directing the traffic back to the ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor, Brefeldin A (BFA) (Cat. No. 203729), the effects appear to be limited to the Golgi, as no effects are detected on endocytic organelles, such as endosomes and trans-Golgi network (TGN). The inhibitory mechanism and target(s) appear to be different from those of BFA, thus making it a valuable alternative that complements BFA in membrane trafficking studies.

A cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells (IC₅₀ = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and redirecting the traffic back to ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor Brefeldin A (BFA; Cat. No. 203729), its effect seems to be limited to Golgi, as no effect can be detected on endocytic organelles, such as endosomes and TGN (trans-Golgi network). Its inhibitory mechanism and target(s) appear to be quite different from those of BFA and thus serves as a valuable alternative that complements BFA in membrane traffic studies.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Golgi ARF1 GTPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 20 µM inhibiting vesicular traffic from ER to Golgi in BSC1 cells

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Feng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA100, 6469.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable