Skip to Content
Merck
HomeMammalian Cell CultureGastrointestinal Organoid Biobank

Gastrointestinal Organoid Biobank

Patient derived organoids (PDOs) are novel in vitro 3D cell models that preserve original tissue physiology and molecular pathology, thus representing a clinically-relevant alternative to traditional 2D cell lines and an effective tool to refine and reduce animal models. PDOs can be derived from adult patient biopsies or resected tissues containing native LGR5+ stem cell populations and cultured within an ECM-rich substrate using specialized organoid media such as L-WRN conditioned media (SCM105). Adult tissue derived organoids are phenotypically and genetically stable in long term culture, presenting more mature phenotypes compared to iPSC-derived organoids. Importantly, PDOs have shown to be able to predict patient clinical responses to chemotherapeutics.

We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.

3dGRO organoids were derived utilizing HUB Organoid Technology. The purchaser of this product shall agree to HUB’s terms of use, which shall be separately acknowledged and accepted by such purchaser, prior to transfer of this product to purchaser.

Biobank Overview

  • 58 Patient Derived Organoid Samples
  • Multiple Genders, Age, and Sex
  • Multiple Tissues/Diseases: Colon, Duodenum, Ilium, Stomach, Rectum, Cancer, Ulcerative Colitis, IBS
  • RNA-Seq Available
  • Low Passage and High Viability
  • Cultured in Optimized 3dGRO L-WRN Conditioned Media
prep-78-c

Prep-78-C (SCC311)

prep-87-c

Prep-87-C (SCC321)

prep-81-c

Prep-81-C (SCC313)

prep-881-cr

14-881-CR (SCC310)

Figure 1. Patient-derived colon organoids from both normal (SCC311, SCC321, SCC313) and diseased patients (rectal adenocarcinoma, SCC310) cultured in L-WRN conditioned media (SCM105) for five passages.

Ordering Information for PDO Biobank

IDCatalog NumberAgeSexOrganDisease Type or Normal
14-881-CRSCC31046MColon (Rectum)Adenocarcinoma (TP53 Pro151Ser, KRAS Gly12Asp,
APC Pro1443fs) (RNA-seq)
Prep 78-CSCC31152FColonNormal (RNA-seq)
Prep 80-CSCC31229FColonNormal (RNA-seq)
Prep 81-CSCC31349FColonNormal (RNA-seq)
Prep 81-DSCC31449FDuodenumNormal (RNA-seq)
Prep 83-CSCC31545FColonNormal (RNA-seq)
Prep 83-DSCC31645FDuodenumNormal (RNA-seq)
Prep 84-CSCC31756FColonNormal (RNA-seq)
Prep 84-DSCC31856FDuodenumNormal (RNA-seq)
Prep 85-CSCC31962FColonNormal (RNA-seq)
Prep 85-DSCC32062FDuodenumNormal (RNA-seq)
Prep 87-CSCC32121MColonNormal (RNA-seq)
Prep 87-DSCC32221MDuodenumNormal (RNA-seq)
Prep 88-CSCC32333FColonNormal (RNA-seq)
Prep 88-DSCC32433FDuodenumNormal (RNA-seq)
Prep 89-CSCC32555MColonNormal (RNA-seq)
Prep 89-DSCC32655MDuodenumNormal (RNA-seq)
18-1417-NSCC327*45MColonNormal (CRC tissue adjacent)
ht-131-CSCC328*58FColonAdenoma
ht-098-CSCC32970FColonNormal (RNA-seq)
ht-099-CSCC33059FColonNormal (RNA-seq)
ht-100-CSCC331*67MColonNormal
ht-101-CSCC33259FColonNormal (RNA-seq)
ht-101-DSCC333*59FDuodenumNormal
ht-102-CSCC334*61FColonNormal
ht-103-CSCC33563MColonNormal
ht-104-CSCC336*51MColonNormal
ht-104-ISCC33751MIleumNormal (RNA-seq)
ht-105-CSCC338*19MColonNormal
ht-105-ISCC33919MIleumNormal (RNA-seq)
ht-118-CSCC34043MColonNormal
ht-119-CSCC341*53FColonNormal
ht-127-SSCC34252FStomachNormal
ht-128-SSCC34360FStomachNormal
ht-129-SSCC34460MStomachNormal
ht-130-SSCC34568MStomachNormal
ht-215-CDSCC346*59FColon descendingNormal
ht-215-CTSCC347*59FColon transcendingNormal
ht-215-DSCC348*59FDuodenumNormal
ht-222-DSCC349*33MDuodenumNormal
ht-180-DSCC35038FDuodenumNormal
ht-185-DSCC35154MDuodenumNormal
ht-188-DSCC352*50FDuodenumNormal margin to UC
ht-205-CSSCC35333FColonNormal Margin to Crohns
ht-205-DSCC35433FDuodenumNormal Margin to Crohns
ht-205-ISCC35533FIleumNormal Margin to Crohns
ht-213-CASCC356*36FColon ascendingNormal margin to UC
ht-213-CDSCC357*36FColon descendingNormal margin to UC
ht-213-CTSCC358*36FColon transcendingNormal margin to UC
ht-213-DSCC359*36FDuodenumNormal margin to UC
ht-214-CSCC359*36FDuodenumNormal margin to UC
ht-214-CSCC360*46MColon J-pouchNormal margin to UC
ht-214-SISCC361*46MSmall Intestine J-pouchNormal margin to UC
ht-227-DSCC362*62MDuodenumNormal margin to UC
ht-230-DSCC363*59FDuodenumNormal margin to UC
ht-205-ISCC364*33FIleumUC
ht-206-CSCC36524FColonCrohns
ht-206-ISCC36624FIleumCrohns
ht-208-CRSCC36723MColon (Rectum)Crohns
ISO38SCC501**N/AN/ASigmoid ColonColorectal Cancer (TP53 Pro151Ser, KRAS Gly12Asp, APC Pro1443fs)
ISO48SCC502**N/AN/ACaecumColorectal Cancer (PIK3CA ∆E542K, CTNNB1 ∆S33C)
ISO49SCC503**N/AN/ALower Sigmoid ColonColorectal Cancer (APC ∆R1450*A1446Lfs*27, TP53 ∆R248Q, KRAS ∆G12D, PIK3CA ∆E542K, FBXW7 ∆R465C)
ISO50SCC504**N/AN/AColon (Rectum)Colorectal Cancer (APC ∆R232*E1286*, TP53 ∆R248Q", KRAS ∆G12D, SMAD4 ∆E526K, SMAD2 ∆S464*)
ISO57SCC505**N/AN/AColon (Upper Rectum)Colorectal Cancer (APC ∆Q978*, TP53 ∆R282W, KRAS ∆G13D)
ISO68SCC506**N/AN/ASigmoid ColonColorectal Cancer (APC ∆Q1096*E1408*, TP53 ∆R248W, KRAS ∆G13D, SMAD4 ∆Q534*)
ISO72SCC507**N/AN/ACaecumColorectal Cancer (APC ∆Q1291*, KRAS ∆G12D, ERBB3 ∆A232V)
* Coming Soon ** Does not require HUB terms of use agreement prior to purchase.

Fig2: Figure 2. Immunocytochemical characterization of human colon organoids (Colon-87, SCC321). Human colon PDOs are positive for colon-specific markers: CA II, CA IV and Mucin5B, posterior hindgut marker: CDX2, stem cell markers: Lgr5 and Sca1 and epithelial markers: TPH-1 and E-Cad.

Fig 2: Immunocytochemical characterization of human colon organoids (Colon-87, SCC321). Human colon PDOs are positive for colon-specific markers: CA II, CA IV and Mucin5B, posterior hindgut marker: CDX2, stem cell markers: Lgr5 and Sca1 and epithelial markers: TPH-1 and E-Cad.

Fig 3: Heatmap of top 30 differentially expressed genes

Fig 3:Heatmap of top 30 differentially expressed genes

Gene expression differences of human iPSCs, iPSC derived colon organoids vs. adult patient derived tissue organoids.

Fig 4: Gene expression differences of human iPSCs, iPSC derived colon organoids vs. adult patient derived tissue organoids.

Adult tissue derived colon (SCC321, Colon-87) and duodenum (SCC320, DUO-85) organoids express SARS-CoV-2 related proteins ACE2 and TMPRSS2.

Fig 5: Adult tissue derived colon (SCC321, Colon-87) and duodenum (SCC320, DUO-85) organoids express SARS-CoV-2 related proteins ACE2 and TMPRSS2.

Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?