GC Analysis of FAMEs in Walnuts on SLB®-IL111
Materials
analytical column
related product
Aldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors
jacket O.D. 37 mm, Overall H 270 mmAldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors
jacket O.D. 37 mm, Overall H 420 mmAldrich® modified Friedrichs condenser with SafetyBarb® tubing connectors
jacket O.D. 54 mm, Overall H 300 mmCONDITIONS
column
SLB-IL111, 100 m x 0.25 mm I.D., 0.20 μm (29647-U)
oven
168 °C
inj. temp.
250 °C
detector
FID, 250 °C
carrier gas
FID, 250 °C
injection
1 μL, split 100:1
liner
4 mm ID FocusLiner w/taper
sample
ground walnuts prepared for analysis per AOCS Official Method Ce 1k-09
Description
Analysis Note
This application illustrates the extraction and GC analysis of walnuts for fatty acids. Walnut is considered a significant plant source of alpha-linolenic acid (ALA). Both ALA and linoleic acid (LA) are the predominant essential fatty acids in humans (1). The samples were prepared using acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). BHT was added as an antioxidant prior to extraction. All samples were concentrated to 1 mL prior to GC analysis. The FAME composition of vegetable oils is normally determined using AOCS Method Ce 1h-05, which designates the use of the a cyanopropylsilicone column (3). The SLB-IL111 is a highly polar column which provides alternate selecitivty and resolution of geometric positional isomers of fatty acid methyl esters. It can be used to complement the method designated column.
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany