- The participation of trimethylammonium in the mouse erythrocyte epitope recognized by monoclonal autoantibodies.
The participation of trimethylammonium in the mouse erythrocyte epitope recognized by monoclonal autoantibodies.
Immunology letters (1981-11-01)
D Serban, J M Pages, A E Bussard, I P Witz
PMID6173316
ABSTRACT
Monoclonal IgM anti-erythrocyte autoantibody produced by a NZB-derived hybridoma has been found to react with trimethylammonium-containing compounds. Such compounds are able to prevent the lysis of bromelain-treated mouse erythrocytes (BrMRBC) by those autoantibodies. Using a column of insolubilized betaine hydrazine (BH) the monoclonal anti-erythrocyte antibody has been specifically retained. Elution of this antibody was accomplished by 0.15 M choline (Ch).
MATERIALS