Skip to Content
Merck

A method for nanofluidic device prototyping using elastomeric collapse.

Proceedings of the National Academy of Sciences of the United States of America (2009-09-01)
Seung-min Park, Yun Suk Huh, Harold G Craighead, David Erickson
ABSTRACT

Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques are limited by the complexity of the devices that can be fabricated, which can only create straight or irregular channels normal to the direction of an applied strain. Here, we report a technique for nanofluidic fabrication based on the controlled collapse of microchannel structures. As is demonstrated, this method converts the easy to control vertical dimension of a PDMS mold to the lateral dimension of a nanochannel. We demonstrate here the creation of complex nanochannel structures as small as 60 nm and provide simple design rules for determining the conditions under which nanochannel formation will occur. The applicability of the technique to biomolecular analysis is demonstrated by showing DNA elongation in a nanochannel and a technique for optofluidic surface enhanced Raman detection of nucleic acids.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
2-Mercaptoethanol, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, for molecular biology, nucleic acid hybridization tested, mol wt 360,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, suitable for plant cell culture, average mol wt 10,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tris-Borate-EDTA buffer, 5× concentrate, powder blend
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, powder, average Mw ~29,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, K 90
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, powder, average Mw ~55,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tris-Borate-EDTA buffer, 5× Concentrate
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tris-Borate-EDTA buffer, working solution
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tris-Borate-EDTA buffer, BioReagent, suitable for electrophoresis, 10× concentrate
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Tris-Borate-EDTA buffer, BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, K 30
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, mol wt (number average molecular weight Mn 360kDa)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, powder, BioXtra, suitable for mouse embryo cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, average mol wt 10,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, average mol wt 40,000
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Polyvinylpyrrolidone, average Mw ~1,300,000 by LS
SKU
Pack Size
Availability
Price
Quantity