- Detection of 500-nucleotide DNA by laser desorption mass spectrometry.
Detection of 500-nucleotide DNA by laser desorption mass spectrometry.
Rapid communications in mass spectrometry : RCM (1994-09-01)
K Tang, N I Taranenko, S L Allman, L Y Cháng, C H Chen
PMID7949335
ABSTRACT
We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.