M8445

Anti-MLH1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-COCA2, Anti-FCC2, Anti-HNPCC, Anti-MGC5172
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 80-85 kDa
• species reactivity: human, mouse, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates
• UniProt accession no.: P40692
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MLH1(4292) ; mouse ... Mlh1(17350) ; rat ... Mlh1(81685)

Description

General description

MLH1 is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BRCA1-associated genome surveillance complex (BASC).

MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is located in chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.

Specificity

Anti-MLH1 (C-terminal) specifically recognizes human MLH1 (80-85 kDa).

Immunogen

synthetic peptide corresponding to amino acids 591-606 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.

Application

Anti-MLH1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation

Biochem/physiol Actions

A hereditary mutation in the MLH1 gene is implicated in nonpolyposis colorectal cancer-2.

MutL homolog 1 (MLH1) has been shown to be involved in promoting colorectal carcinogenesis.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves