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Merck

L6170

Sigma-Aldrich

β-Lactamase

recombinant, expressed in E. coli

Synonym(s):

Carbapenemase, Cephalosprinase

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5000 MG
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5000 MG
€54.30

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54

€54.30


Please contact Customer Service for Availability

To order products, please contact your local dealer.

recombinant

expressed in E. coli

Quality Level

form

powder

specific activity

≥20 U/mg (with cephalosporin C)
≥400 U/mg (with benzylpenicilin)

storage temp.

2-8°C

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I1882I4011I0516
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400

product line

BioReagent

product line

BioXtra

product line

-

product line

BioReagent

form

powder

form

lyophilized powder

form

powder

form

solution

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria.[1] There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis.[2] Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs).[3] As close to 900 types of β-Lactamases are produced by microbes.[4]

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression[5]. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
β-Lactamase has been used in coumarin-cephalosporin-fluorescein[4]-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells[6] and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.[7]

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring[5]. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg

Unit Definition

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.

Physical form

Lyophilized powder containing sodium chloride and potassium phosphate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Shaikh S, et al.
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