MAB1664
Anti-VEGF Receptor-1 Antibody
Chemicon®, from mouse
Synonym(s):
FLT-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FLT1(2321)
Specificity
Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
1998; Sait et al., 1995; deVries et al., 1992).
SPECIES REACTIVITIES:
Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.
Immunogen
Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1.
Application
Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Western blot: 0.5-2.0 μg/mL. MW = 180 kDa.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Flow cytometry: Dilute the antibody to 25 μg/mL and use 10-20 μL antibody for 1-2 x 10E5 cells in a total volume of 200-250 μL. Visualize with appropriate secondary antibodies.
ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
Purified from Ascites. Liquid in PBS and 5% Trehalose.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C de Vries et al.
Science (New York, N.Y.), 255(5047), 989-991 (1992-02-21)
The fms-like tyrosine kinase (Flt) is a transmembrane receptor in the tyrosine kinase family. Expression of flt complementary DNA in COS cells conferred specific, high-affinity binding of vascular endothelial growth factor, also known as vascular permeability factor (VEGF-VPF), a factor
H Matsushime et al.
Japanese journal of cancer research : Gann, 78(7), 655-661 (1987-07-01)
We have isolated a human genomic DNA (designated human frt) cross-hybridizing with the v-ros oncogene of UR2 sarcoma virus. Sequencing analysis of this fragment revealed that this sequence contains a 123-base-pair exon-like structure surrounded by consensus sequences of splice acceptor
Isolation and characterization of a newly identified endothelial cell mitogen produced by AtT-20 cells.
Plouet, J, et al.
The Embo Journal, 8, 3801-3806 (1989)
K Choi et al.
Oncogene, 9(4), 1261-1266 (1994-04-01)
A mouse gene encoding a receptor tyrosine kinase, designated Embryonic receptor kinase (EmRK2), was isolated from embryoid bodies (EBs) generated by differentiating embryonic stem (ES) cells in culture for 6 days. Sequence analysis of EmRK2 cDNA clones predicts a receptor
H Finnerty et al.
Oncogene, 8(8), 2293-2298 (1993-08-01)
A wide range of growth and differentiation processes are regulated by the signalling of receptor tyrosine kinases (RTKs). We have developed a nested polymerase chain reaction (PCR) procedure with degenerate primers, and used it to identify RTKs expressed in murine
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