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368059-M

Millipore

Granzyme B Substrate II, Fluorogenic

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.

Synonym(s):

Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II

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About This Item

Empirical Formula (Hill Notation):
C37H42F3N5O13
Molecular Weight:
821.75
UNSPSC Code:
12352200

Quality Level

Assay

≥97% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white

solubility

DMSO: 1 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

storage temp.

−20°C

General description

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.
A fluorogenic substrate for the detection of granzyme B. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Biochem/physiol Actions

Primary Target
Fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Ile-Glu-Thr-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Karahashi, H., and Amano, F. 2000. Biol. Pharm. Bull. 23, 140.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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