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204882

Sigma-Aldrich

Complement C2, Human

Complement C2, Human, is a native, single chain glycoprotein present. C2 is cleaved by the activated C1s into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment.

Synonym(s):

C2 Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

Quality Level

100

Assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥4×105 C2H50 units/mg (>=70% of the C2 activity in normal human serum on a mg/mg basis)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

C1, C3, C4, C5, IgG, IgA, IgM, albumin, Factor B, or hemopexin, trace

shipped in

wet ice

storage temp.

−70°C

General description

Native, human C2 complement component. Single chain glycoprotein present in normal human serum at concentration of 20 µg/ml. Classical pathway activation results in assembly of the C1,C4b complex on target surfaces. Subsequent to binding of the C4b component to this complex, C2 is cleaved by the activated C1s subcomponent of C1 into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment. The smaller C2b fragment is released while the larger C2a fragment remains associated with the C1,C4b complex to form the C4b,C2a classical pathway C3 convertase enzyme. C2 concentration is based on functional activity measurements relative to a normal human serum standard pool.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 6.0.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Attwood, J.T., et al. 2001. Acta Paediatr.90, 99.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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