OligoArchitect™ Consultative

For more complex and demanding applications, utilize our consultative service to ensure the success of your assay. With personal consultation from our expert, technical support scientists, your request, including all sequences and data analysis, will be MIQE compliant and returned to you within 24-48 hours. If required, you will also receive follow-up assay optimization, data analysis assistance, and troubleshooting support.

Specifications

Design Modules	PCR and qPCR Applications
SYBR®Green I Primers Dual-Labeled Probes1 Molecular Beacons2 LightCycler® Probes3 Scorpions® Probes	Traditional PCR SNP detection Allele discrimination Pathogen detection Multiplexing Viral load quantification Gene expression analysis Gene copy determination Endpoint genotyping Methylation analysis High-resolution melting analysis Transcript detection across exon junctions Haplotyping Northern blotting Southern blotting
Other Applications
FISH (fluorescence in situ hybridization)
Modifications
Add Locked Nucleic Acid to any of the above design modules4

¹ Also known as hydrolysis probes, which are used in the 5’ nuclease assay
² Also known as hybridization probes
³ Also known as dual-hybridization probes
⁴Locked Nucleic Acid in oligonucleotides other than qPCR probes is available in all countries except for the United States.

Our consultative service uses Beacon Designer™ from Premier Biosoft International. Blastn and mfold are used to determine assay specificity.