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column temp.	30 °C
mobile phase	[A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)
flow rate	0.2 mL/min
pressure	1150 psi (79 bar)
sample	10 ng/mL in mobile phase
injection	1 µL
detector	MS, ESI(+), MRM mode

\r\n",":type":"cms-commons/components/content/table"},"table_1183631280":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Analysis Note	The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis^® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.
Categories	Analytical Chromatography, Immunosuppressants
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
suitability	application for HPLC

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