Plasmid DNA Preparation

Product lists for commonly used protocols from Cold Spring Harbor Protocols

Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation

Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas

ABSTRACT

Plasmid DNA may be isolated from large-scale (500 mL) bacterial cultures by treatment with alkali and SDS. The resulting DNA preparation may be further purified by column chromatography or centrifugation through CsCl-ethidium bromide gradients.

Products Available for this Protocol

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