GC Analysis of Pyrazines in Peanut Butter on SUPELCOWAX^® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

Table 1

Sample/Matrix	5 g peanut butter in 40 mL vial
Desorption Process	270 °C for 5 min
Extraction	Headspace, 30 min at 65 °C in heating block
SPME Fiber	Divinylbenzene-Carboxen-polydimethylbenzene StableFlex™ (57328-U)
Column	SUPELCOWAX® 10, 30 m x 0.25 mm, 0.25 µm (24079)
Oven	40 °C (5 min) to 230 °C at 4 °C/min
Carrier Gas	Helium, 30 cm/sec
Sample	Peanut butter
Injection	Splitless/split, closed 0.5 min, 270 °C, with 0.75 mm liner
Liner	0.75 mm
Inj. Temp.	270 °C
Detector	Ion trap mass spectrometer, m/z =30-350 at 0.6 sec/scan. Selected ions used for quantitation.

Description

Table 2

Analysis Note	In this application, SPME was used for the analysis of volatiles in multifloral honey using a headspace method. Headspace SPME (HS-SPME) using a Carboxen/PDMS/DVB fiber coupled with gas chromatography-mass spectrometry (GC/MS) was used for the characterization of the volatile fraction of honey samples. Using SPME it was possible to obtain and distinguish the chemical fingerprints of different types of honey. The relatively polar SUPELCOWAX® 10 GC column provides excellent separation of these polar compounds.
Categories	Analytical Chromatography, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Volatiles
Featured Industry	Agriculture Food and Beverages
Legal Information	SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes	Complementary Products: Syringes Vials Gas Purification and Gas Management Inlet Liners, Glass Wool, and Specialized Hand Tools Septa and Specialized Hand Tools Column Ferrules, Nuts, and Specialized Hand Tools
Suitability	Application for SPME, Application for GC

Analyte

• 1-Methylpyrrole
• 2,3-Diethylpyrazine
• 2,3-Dimethylpyrazine
• 2,5-Dimethylpyrazine
• 2,6-Diethyl pyrazine
• 2,6-Dimethylpyrazine
• 2-Allyl-5-methylpyrazine
• 2-Ethenyl-5-methylpyrazine
• 2-Ethenyl-6-methylpyrazine
• 2-Ethyl pyrazine
• 2-Ethyl-3,5-dimethyl pyrazine
• 2-Ethyl-3-methyl pyrazine
• 2-Ethyl-5-methyl pyrazine
• 2-Ethyl-6-methyl pyrazine
• 2-Methyl-5-isopropylpyrazine
• 2-Methyl-5-propylpyrazine
• 2-Methyl-6-cis propenylpyrazine
• 2-Methylpyrazine
• 3,5-Diethyl-2-methyl pyrazine
• 3-Ethyl-2,5-dimethylpyrazine
• 3-Methylbutanal
• 4-Methylpentene-2-one
• 5-Methyl-2-propylpyrazine
• Carbon disulfide
• Dimethyl disulfide
• Heptanal
• Hexanal
• Pentanal
• Trimethyl pyrazine