UHPLC/MS Analysis of Antineoplastic and Antidepressant Drugs in Plasma on Titan C18 after SPE using HybridSPE^®-PLus

sample/matrix

rat plasma

SPE well plate

HybridSPE-PLus 96-Well Plate, 50 mg/2 mL per well (575659-U)

sample preparation

final analyte concentration 50 ng/mL

sample addition

400 μL of 1% formic acid acetonitile:spiked plasma (3:1) per well

column

Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 90:10 (v:v) acetonitrile:water

gradient

35 to 90% B in 1.5 min, held at 90% B for 0.4 min

flow rate

0.4 mL/min

pressure

3582 psi (247 bar)

column temp.

35 °C

detector

TOF/MS

injection

1 μL