LC/MS/MS Analysis of Drugs of Abuse: Generic Screening on Ascentis® Express F5
Materials
분석 컬럼
표준
Benzoylecgonine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Fentanyl solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Hydrocodone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Morphine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(±)-Methamphetamine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(±)-MDMA solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Meperidine solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Normeperidine solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Oxycodone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®(+)-Propoxyphene solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase
[A] 5 mM ammonium formate water pH 4.0 formic acid; [B] 5 mM ammonium formate (95:5 acetonitrile:water)
gradient
20% B for 0.5 min; to % B in 5.5 min; held at 100% B for 3 min
flow rate
0.3 mL/min
pressure
2538 psi (175 bar)
column temp.
35 °C
detector
MS, ESI(+), TIC, m/z 100-1000
injection
1 μL
sample
each compound 1000 ng/mL in 40:60 (water:methanol)
설명
분석 메모
Shown here is a method to rapidly screen a sample for a variety of abused substances using an Ascentis Expres F5 column. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany