Improved HPLC Selectivity of Discovery^® BIO Wide Pore RP Phases

column

Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 μm particles vs. competitive wide pore C18 column of same dimensions (568222-U)

mobile phase

[A] 0.1% TFA in 80:20 water:acetonitrile; [B] 0.1% TFA in 66:34 water:acetonitrile

Gradient of 0 to 100% B in 14 min. after 1 min. delay

gradient

0 to 100% B in 14 min. after 1 min. delay

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

10 μL

sample

peptide mixture (Sigma P2693), ~0.25 μg each peptide in 0.1% TFA