HPLC Analysis of Tramadol Enantiomers on Astec^® CYCLOBOND I 2000 DMP

column

CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)

mobile phase

[A] 0.1% triethylamine acetate, pH 5.0; [B] acetonitrile; (80:20, A:B)

flow rate

1.2 mL/min

column temp.

23 °C

detector

UV, 254 nm

injection

5 μL

sample

5 mg/mL in methanol