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HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

Materials

분석 컬럼

제품 번호
설명
가격

581512-U

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

표준 물질

제품 번호
설명
가격

M8378

Maltotriose

≥90% (HPLC)

84097

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

Lactose Monohydrate

analytical standard

G0350500

Glucose

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375025

Maltose monohydrate

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

설명

분석 메모

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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