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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85) application for HPLC

Materials

분석 컬럼

제품 번호
설명
가격

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

표준

제품 번호
설명
가격

M8378

Maltotriose

≥90% (HPLC)

84097

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

Lactose Monohydrate

analytical standard

G0350500

Glucose

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375025

Maltose monohydrate

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

설명

분석 메모

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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