HPLC Analysis of Proteins on Proteomix^® SCX Columns, Effect of Particle Size of Resolution

column

Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)

column

Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)

mobile phase

(A) 20 mM sodium phosphate buffer, pH 6.0, (B) A + 1.0 M NaCl

gradient

0-25 min from 0-75% B, 15 min prewash

flow rate

0.75 mL/min

column temp.

Ambient

detector

214 nm

injection

5 μL

sample

each 1 mg/mL