HPLC Analysis of Peptides from BSA on Proteomix^® SCX-NP3, Volatile Buffer

column

Proteomix SCX-NP1.7, 3 cm x 2.1 mm I.D., 1.7 μm particles (Z777142)

mobile phase

(A) 10 mM sodium phosphate, pH 2.5; (B) A + 1 M NaCl; (C) acetonitrile

gradient

90% A, 0% B, 10% C held for 1.5 min; to 40% A, 50% B, 10% C in 18.5 min

pressure

5511 psi (380 bar)

detector

UV, 214 nm

sample

trypsin digested bovine serum albumin (BSA)

flow rate

0.35 mL/min