HPLC Analysis of Peptides and Proteins on Proteomix^® SCX-NP1.7, 3 cm x 4.6 mm I.D. Column

column

Proteomix SCX-NP1.7, 3 cm x 4.6 mm I.D., 1.7 μm particles (Z777145)

mobile phase

(A) 20 mM sodium phosphate, pH 6.0; (B) A + 1.0 M NaCl

gradient

0 to 50% B in 5 min; 15 min prewash

flow rate

2 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL