HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH
Materials
분석 컬럼
제품 번호
설명
가격
CONDITIONS
column
SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 μm particles (58318)
mobile phase
[A] water, [B] methanol
gradient
55 to 100% B in 5 min; held at 100%B for 10 min
flow rate
1.5 mL/min
column temp.
35 °C
detector
UV, 254 nm
injection
5 μL
sample
EPA 610 mix
설명
분석 메모
PAHs are traditionally evaluated using an acetonitrile/water gradient. Recently customers are looking for alternatives to this method using a methanol/water gradient due to the risk of an another acetonitrile shortage, solvent costs, and toxicity to the environment. This analysis demostrates the separation using a methanol gradient.
법적 정보
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC