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HPLC Analysis of mAb 321 on Proteomix® SCX NP5 versus Competitive SCX using pH and Salt Gradients

HPLC Analysis of mAb 321 on Proteomix® SCX NP5 versus Competitive SCX using pH and Salt Gradients application for HPLC

Materials

분석 컬럼

제품 번호
설명
가격

Proteomix® SCX-NP5 Strong Cation Exchange Column

L × I.D. 25 cm × 4.6 mm, 5 μm particle size, PEEK

CONDITIONS

column

Proteomix SCX-NP5, 25 cm x 4.6 mm I.D. (PEEK hardware), 5 μm particles (Z777164)

mobile phase

(A) 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; (B) A + 0.5 M NaCl, pH 10.5

flow rate

0.8 mL/min

detector

UV, 280 nm

injection

20 μL

sample

MAb 321 (5 mg/mL)

설명

법적 정보

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