HPLC Analysis of Ferredoxin NADP Reductase on TSKgel^® Phenyl-5PW

column

TSKgel Phenyl-5PW column, 7.5 cm × 7.5 mm I.D., 10 μm particles (807573)

mobile phase

60 min decreasing linear gradient from 1.5M to 0M ammonium sulfate in 0.1M sodium phosphate buffer, pH7

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

3mg (crude) or 0.2mg (standard)