HPLC Analysis of Fat Soluble Vitamins on Ascentis^® Express RP-Amide, Methanol Mobile Phase

column

Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)

mobile phase

methanol

flow rate

1.0 mL/min

pressure

1653 psi (114 bar)

column temp.

30 °C

detector

UV, 280 nm

injection

5 μL

sample

0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol