HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentisยฎ Express RP-Amide (UV Detection)
Materials
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Ascentisยฎ Express RP-Amide, 2.7 ฮผm HPLC Column
2.7 μm particle size, L ร I.D. 5 cm ร 4.6 mmCONDITIONS
column
Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)
mobile phase
[A] water; [B] acetonitrile
gradient
50 to 100% B in 10 min
flow rate
1.0 mL/min
column temp.
35 ยฐC
detector
UV, 280 nm
injection
10 μL
sample
50 mg/L of each in 50:50, water: acetonitrile
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๋ถ์ ๋ฉ๋ชจ
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds
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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany