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Merck

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentisยฎ Express RP-Amide (UV Detection)

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection) application for HPLC

Materials

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Ascentisยฎ Express RP-Amide, 2.7 ฮผm HPLC Column

2.7 μm particle size, L ร— I.D. 5 cm ร— 4.6 mm

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Alkylphenol Internal Standard Mix 7-solution for DIN EN ISO 18857-2

in acetone, analytical standard

Alkylphenol Kit for DIN EN ISO 18857-2

analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)

mobile phase

[A] water; [B] acetonitrile

gradient

50 to 100% B in 10 min

flow rate

1.0 mL/min

column temp.

35 ยฐC

detector

UV, 280 nm

injection

10 μL

sample

50 mg/L of each in 50:50, water: acetonitrile

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This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds

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Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany